TriFast II

Product Description

TriFast II is a complete ready to use mono-phasic reagent for the simultaneous isolation of RNA, DNA and proteins from solid and liquid samples of human, animal, plant, yeast, bacterial and viral origin. The isolation method for RNA, DNA and proteins is built on the well-known single step liquid phase separation, with additional modifications to improve the purity of the RNA, DNA and proteins. This method has been shown to yield non-degraded nucleic acids (RNA and DNA) from small and large samples and allow the isolation of large and small RNA-species (0.1 - 15 kb) with high purity. Also it makes possible to process a large number of samples simultaneously.
TriFast II includes phenol and guanidinium thiocyanate in a monophasic solution. After addition of chloroform, the homogenate separates into three phases upon centrifugation. RNA will be selectively retained in the aqueous phase, while DNA and proteins stay respectively in the organic phase and interphase and are processed separately, resulting in RNA, DNA and protein of excellent quality in high yield. Simultaneous purification of DNA is an effective way to normalize RNA yields from sample to sample.

Features

• Purification: RNA, DNA and proteins
• Format: Reagent + phenol/chloroform
• Starting materials: Cells and tissue
• Staring Quantity: Scalable
• Expected Yield: Up to 7 μg RNA/mg tissue
• RNA isolation time: Appr. 1 hour

Protocol At-a-Glance

• Homogenization and lysis of the sample with TriFast II
• Centrifugation
• Addition of chloroform and RNA extraction from the upper phase
• RNA precipitation and resuspension
• DNA extraction from the interphase/organic phase
• DNA precipitation and resuspension
• Protein extraction from the ethanol/phenol phase
• Protein purification

Expected Yields

Note

TriFast II replaces our TriSure products (BIO-38032-BL, BIO-38033-BL), which are no longer available. TriFast offers 100% equivalent performance.