Constitutive (CAGs promoter) EEV cloning and expression vector

Enhanced Episomal Vectors (EEV) for sustained, non-integrating transgene expression

Product Description

With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. 

Use the CAGs-MCS EEV Vector for constitutive expression in most cell types including primary cells and stem cells with the strong CAG promoter. A Multiple Cloning Site (MCS) is featured to insert a cDNA, microRNA, etc. sequence into the EEV vector as well as the EBV oriP origin and the EBNA1 factor for propagating the episome for sustained transgene expression.

For inducible EEV expression we offer the Cumate-Inducible (CuO) EEV cloning and expression vector.

Services

Custom EEV Cloning and Plasmid DNA Production can be offered. 

For Academic and Non-Profit Institutions

Researchers at academic and non-profit institutes are granted full access to purchasing the EEV cloning vectors and reporters. Full sequence information is provided upon proof of purchase of EEV vectors. Please contact us for sequence information.

For Commercial Customers

 Due to licensing restrictions, cloning EEV vectors (e.g. EEV600A-1-SBI and EEV610A-1-SBI) are not available for purchase by for-profit and commercial customers directly. Instead, these are available through SBI’s custom EEV cloning and production services (see above). SBI then provides the commercial customer with the desired amount of ready-to-use EEV custom construct DNA.