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Oxaloacetate Colorimetric Assay Kit
Specifications | |
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Research Area: | Cell Metabolism |
Assay Category: | Tricarboxylic Acid Cycle/Krebs Cycle/Citric Acid Cycle |
Sample Type: | animal tissue |
Detection Method: | Microplate reader |
Sensitivity: | 0.017 mmol/L |
Detection Range: | 0.017-1 mmol/L |
Product Information
Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.
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