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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Specifications | |
---|---|
Research Area: | Cell Metabolism |
Assay Category: | Liver Function |
Sample Type: | serum, plasma, animal tissue, cells, cell culture supernatant |
Detection Method: | Microplate reader |
Sensitivity: | 0.75 IU/L |
Detection Range: | 0.75-72.30 IU/L |
Product Information
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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- Catalog Number
E-BC-K235-M-500Assays-ELS - Supplier
Elabscience - Size
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