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Lactate Dehydrogenase (LDH) Activity Assay Kit
Specifications | |
---|---|
Research Area: | Cell Metabolism |
Assay Category: | Metabolic Enzymes |
Sample Type: | serum, plasma, hydrothorax, tissue, cells |
Detection Method: | Microplate reader |
Sensitivity: | 6 U/L |
Detection Range: | 6-500 U/L |
Product Information
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Product Citations
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- (2023) Targeting the mechanism of IRF3 in sepsis-associated acute kidney injury via the Hippo pathway INTERNATIONAL IMMUNOPHARMACOLOGY
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E-BC-K046-M-500Assays-ELS - Supplier
Elabscience - Size
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