Dual Luciferase Reporter Gene Luminescence Assay Kit (Flash Type)

Product Description

Detection priciple: Dual luciferase reporter gene uses luciferin to detect the luminescence reaction produced by luciferin substrate. The detection system includes two parts: firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data.

The reaction principle of this test box is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferase and coelentin are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence instrument.