Double-Quenched BHQnova Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ (Black Hole Quencher) and internal Nova quencher

Product Description

BHQnova™ Probes are a double-quenched probe format that improves quenching efficiency and signal release. This quencher configuration is particularly suited to longer probes sequences, typically 25 bases or longer, which otherwise may suffer from poor quenching efficiency as traditional end-labeled probes.

Design Recommendations for BHQnova probes

BHQnova probes provide the greatest benefit for probe designs that are 25 bases or longer. For those shorter than 25 bases, a standard (dual labeled) BHQ® probe may be perfectly satisfactory.

BHQnova probes contain the internal Nova quencher modification by default. This second quencher is always incorporated at the same position, and so there is no need to designate the internal modification within the sequence itself. When using the web order form, as below, the Nova quencher will automatically be incorporated upon adding to the shopping cart. When submitting an Excel file, select “BHQ-1 nova” within the field for 3’ Modification.

BHQnova probes are perfectly compatible with other probe formats within multiplexed configurations. Barring any sequence interactions, you can combine BHQnova probes with standard BHQ probes or BHQplus® probes, all within the same reaction.

For available fluorophores please check the BHQnova Product Listing. Suitable quenchers can be viewed in the Fluorophore and BHQ Selection Chart.