Custom Lentiviral Dual sgRNA Expression Construct for Two-Vector CRISPR System (plasmid)

Easily get the CRISPR KO, CRISPRi, or CRISPRa sgRNA Constructs you want

Product Description

The CRISPR/Cas9 system can be used for gene knockout (KO), knockdown, activation or to initiate knock-ins in vivo or in vitro by using a combination of an sgRNA (single-guide RNA) together with a Cas9 nuclease. Some examples of custom constructs include:

Constitutive or tet-inducible sgRNA constructs designed for CRISPR KO, CRISPRa, or CRISPRi.
All-in-one constructs with sgRNA and Cas9 or single-vector sgRNA-only formats.
Complete panels of Cas9 and dCas9-hybrid (e.g., dCas9-KRAB, dCas9-VPH, dCas9-VPR, etc.) expression constructs

Cellecta's improved sgRNA design enables better CRISPR Knockout Screen results. Learn more about the HEAT-Modified sgRNA Design.

We offer both, a Two-Vector System using sgRNA-only constructs together with Cas9 or dCas9 variants on a separate construct and Single-Vector sgRNA-Cas9 constructs (one construct expressing both the Cas9 gene and your sgRNA). Just provide the gene information and Cellecta will select the optimal guide designs. For Two-Vector System please choose a construct from Celecta's range of CRISPR Cas9 Expression Constructs with various selection markers.

Cellecta Crispr Two Vector Prsg16 And Pr Cmv Cas9 Hygro

Fig.1. sgRNA-only construct and Cas9 expression construct

Performance Data

CRISPR sgRNA constructs targeting GFP were built using the Two Vector and Single Vector CRISPR systems and used to transduce cells that had been previously engineered to stably express GFP. Both CRISPR systems provide at least 80% knockout of GFP, but the two-vector system performed better with over 90% knockout. The enhanced performance is due to pre-selection of cells expressing high levels of Cas9 before transduction with the GFP sgRNA constructs.

Cvcrc Crispr Copgfp Knockout 293 Cells 553

Fig.2. Several sgRNAs targeting the copGFP gene were designed and individually transduced into HEK293 cells stably expressing copGFP. After 9 days, cells were imaged for GFP fluorescence. For each sgRNA tested, GFP expression was abolished in at least 70% of transduced cells.

Cvcrc Crispr Copgfp Ko Graph

Fig.3. After 9 or 14 days. cells were analyzed by FACS. GFP expression was abolished in at least 70% of the analyzed cells after day 9 and at least 90% after day 14.

Cvcrc Crispr 2 Vector Tet Inducible Knockout 2000x