Single Lentiviral sgRNA/shRNA Packaging Service (non-Cas9 construct)

Product Description

Cellecta can provide any of its lentiviral constructs, libraries, or other non-Cellecta lenti-constructs (on a case-by-case basis) as ready-to-transduce, pre-packaged, VSV-g pseudotyped lentiviral particles. These lentiviral preparations can be directly added to cells in culture. The lentiviral particles fuse with the cell membranes and the lentiviral RNA enters the cell, is reverse-transcribed, and integrated into the cell’s DNA.

Cellecta‘s lentiviral preparations are titered by assessing transduction rates of HEK293 cells to assess the number of functionally active (i.e., infection-competent) lentiviral particles. We do not simply measure the amount of viral coat proteins (e.g., p24 assay) or viral DNA. This method ensures more accurate titers and consistent efficacy across different batches.

We can provide various amounts of virus depending on your needs. Packaging scales are based on the total number of viral particles you want, regardless of the volume. For example, we can provide 100 million (10⁸) transduction units (TU) in 10 ml of 10⁷ TU each (i.e., 10⁷ TU/ml), or in 1 ml of 10⁸ (i.e., 10⁸/ml). With both variations, we provide the same amount of total virus so the price does not change. Typically, we provide titers of ca. 2 x 10⁷ to 5 x 10⁷ TU/ml. However, if you would prefer higher or lower concentrations, we will do our best to accommodate you.