Adeno-Associated Virus (AAV) Service GeneCopoeia

AAV Production Service using AAVPrime™ System

Product Description

GeneCopoeia offers a high quality AAV Production Service using the AAVPrime™ Technology. The system uses a helper virus-free system for the safe preparation of high titer AAV. HEK293T cells are co-transfected with three plasmids encoding the factors necessary for recombinant AAV packaging (see figure below). The AAV particles are then either membrane purified or purified by two-phase partitioning for high-titer AAV ready for in vivo animal use.

The destination vector carries your gene of interest (GOI) and two ITRs. You can choose from a variety of destination vectors carrying promoters including CMV, EF1a, CAG, CBh and other tissue-specific promoters, and reporter gene GFP for optimal gene expression and detection. The plasmid that carries the Rep and Cap genes from wild type AAV and the plasmid that carries adenovirus VA, E4 and E2A genes required for efficient AAV production are co-transfected with the destination vector for AAV packaging.

We would be happy to discuss the service options with you. Please contact us if you are interested.

AAV Serotype Choices

GeneCopoeia provides AAV for all the commonly-occurring AAV serotypes, including AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, AAV13, AAV-DJ, AAV-DJ/8, MyoAAV, AAV-MG, AAV-PHP.eB and AAV-BI30, AAV-PHP.S, AAV2.7m8, AAV2-QuadYF, AAV2-retro. The intensity of gene expression and tissue tropism depends highly on the AAV serotype used in different tissues.

Different serotypes differ in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the table below to compare the essential differences between different serotypes. 

AAV Serotypes Available

Two AAVPrime™ Serotype Testing Kits are available for some of the serotypes. They are ideal for testing the infection efficiency of the different AAV serotypes in different cell types. AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit contains 9 premade GFP-expressing AAV in serotypes 1, 2, 3, 6, 7, 8, 9, DJ and DJ/8. AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit Plus contains 12 premade GFP-expressing AAV in serotypes 1-10, DJ and DJ/8. All AAV particles are offered at 5x 10¹² GC/ml, in 25 µl stocks, except serotypes 4 and 10 are offered at 5x 10¹¹-10¹² GC/ml.

Performance Data

Figure 2. AAV of different serotypes carrying GFP were used to transduce the H1299 cell line as shown in the figure above. Fluorescent images (GFP) show that cells are transduced with AAV-GFP.