Adeno-Associated Virus (AAV) Cloning & Packaging Services
Adeno-Associated Virus (AAV) Production Services
The easy way to high quality ready-to-transduce AAV particles
Adeno-associated virus (AAV) products are the ideal tools for expressing or modifying genes in a broad range of cell and tissue types, especially in vivo, with high efficiency and enhanced safety.
Advantages of the AAV System
- Used as a promising candidate for gene therapy
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
BioCat cooperates with partners who have many years of experience with AAV production. Using their services can greatly enhance your research results. A complete service including design, cloning and viral packaging of your custom AAV construct can be ordered as well as only cloning or packaging steps. The service is available for a range of AAV serotypes (1-11, DJ, DJ8).
AAV Production
Generation of rAAV vectors requires 4 key components. Plasmids acting in-trans and the transgene acting in-cis. This modern AAV production system allows viral packaging without potentially pathogenic helper adenovirus.
- a plasmid containing the AAV Rep and Cap genes required for capsid formation and replication,
- a plasmid containing the necessary adenovirus helper genes,
- a cassette containing the transgene enclosed by two inverted terminal repeats (ITR), and
- a viral packaging cell line.
Get in Contact
We would be happy to discuss your project and help to find out which service components have to be selected to meet your requirements. Please get in touch with us.