SensiFAST SYBR No-ROX One-Step Kit

Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.

• Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
• Robust – reliable detection of RNA targets from a broad range of sample types
• Fast – delivers reproducible, accurate assay results in as little as 40 minutes

Product Description

The SensiFAST™ SYBR^® No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.

An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.

The SensiFAST SYBR^® No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

Applications

• Gene expression analysis
• Pathogen detection
• RNA viral pathogen detection
• Genetic profiling
• miRNA profiling / quantification
• Genetically modified organisms (GMO) characterization

Unparalleled performance under fast conditions

Human ß-actin gene was amplified under fast thermal cycling conditions from a 10-fold serial dilution of RNA using SensiFAST SYBR One-Step Kit (red) and a similar kit from Supplier A (blue). SensiFAST delivers superior sensitivity and reproducibility, across 5 orders of magnitude, as judged by the lower and more consistent Ct values.

SensiFAST Products Customer Review

SensiFAST SYBR No-ROX One-Step Kit Customer Review