Minute Nuclei and Cytosol Isolation Kit for Adipose Tissues (20 Tests)

Product Description

Adipocytes are the major energy storage sites in the body and they also have critical endocrine functions. Therefore, understanding the development and function of adipocytes are essential to understanding metabolic homeostasis under physiological and pathological conditions.

Fractionation of cellular components into nucleus and cytosolic fraction is a common practice in the lab. However, when it comes to adipocytes, separation of these two fractions are much more difficult because of high concentration of lipid droplet and low protein content of adipocytes. Methods reported in the literature are tedious and time consuming and as much as 50 grams of tissue are required. This kit provides a very rapid and simple method for obtaining high purity nuclei from adipose tissues and most important of all, only milligram amounts of tissues are required making it possible to isolate nuclei and cytosol from small animal and biopsy samples. The intact nuclei isolated have many applications such as single nucleus RNA sequencing, SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications.

Nuclei isolated from white adipose tissues of rat, chicken, pig and sheep. The nuclei were stained with trypan blue.

SDS-PAGE and Western blot of rat white adipose proteins, T, total protein, N, nuclear protein and C, cytosol proteins.