Custom Capture module, Tier 2

Custom Capture module, Tier 2: 0.5 Mb – 2.99 Mb

Product Description

The Arima Capture-HiC+ platform technology enables researchers to characterize the spatial, 3-dimensional properties of genomic regions of interest. Researchers now have the ability to compare and contrast the regulatory landscape of different tissues/samples with Promoter Capture, detect different classifications of structural rearrangements at high-resolution with Region Capture, and assess the impact of non-coding variants on the coding regions of the genome with Variant Capture. This separate Custom Capture module can be used together with the Arima HiC+ Kit (A510008-ARI) and the Arima Library Prep kit (A303011-ARI) as describedn in the Arima Capture-HiC Workflow below.

Arima Capture-HiC Workflow:

Arima Capture-HiC is an experimental workflow that captures the sequence and structure (threedimensional conformation) of genomes. Arima Capture-HiC has been successfully performed on a wide-range of species from the plant and animal kingdoms. Chromatin from the sample source (tissues, cell lines, or blood) is first crosslinked to preserve the genome sequence and structure. Using the Arima HiC+ Kit the crosslinked chromatin is then digested with a restriction enzyme (RE) cocktail. The 5’-overhangs are then filled in, causing the digested ends to be labeled with a biotinylated nucleotide. Next, spatially proximal digested ends of DNA are ligated, capturing the sequence and structure of the genome. The ligated DNA is then purified, producing pure proximally-ligated DNA. The proximally-ligated DNA is then fragmented, and the biotinylated fragments are enriched. The enriched fragments are then subjected to a custom library preparation protocol utilizing the Arima Library Prep Module. The interactions of interest are enriched using biotinylated RNA probes from the Arima Custom Panel Tier 2.

Please contact info@biocat.com for help designing custom probes.