Soil DNA Isolation Plus Kit

Product Description

• Rapid and convenient method to detect microorganisms in soil samples
• Process all soil types
• Remove organic substances using the included OSR Solution
• Remove all humic acid from DNA samples
• Fast and easy processing using a rapid spin-column format
• Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae

Norgen's Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

Application Note

Norgen’s Improved Soil DNA Isolation Plus Kit Produces Yields and Quality Comparable With a Top Competitor

Kit Performance

Figure 1. Comparison of DNA Yield from Top Soil and Clay Samples. Norgen's Soil DNA Isolation Plus Kit (Cat. 64000-NB) and Competitor Ms kit were used to isolate DNA from 250 mg of top soil and clay samples. Following isolation, 10 µL from each 100 µL elution was loaded on 1% TAE agarose gel. Lane M: Norgen's HighRanger 1kb DNA Ladder.

Figure 2. Comparison of DNA Concentration Isolated from Top Soil and Clay Samples. Norgens Soil DNA Isolation Plus Kit (Cat. 64000-NB) and Competitor Ms kit were used to isolate DNA from 250 mg top soil and clay samples. Norgens kit showed higher DNA concentrations for both samples in comparison to the competitor kit.

Figure 3. High Quality DNA confirmed by Real-time PCR. Soil DNA was isolated from 250 mg of clay (containing low humic acids) and top soil (containing high humic acids) samples using Norgen's Soil DNA Isolation Plus Kit and Competitor M's Kit. DNA quality was confirmed by Real-time PCR using 4 µL of soil DNA (total PCR reaction volume was 20 µL) to detect 16s rDNA from different soil samples. The earlier Ct value with Norgen's DNA samples (red lines) compared to Competitor M's samples (green lines) indicated a higher quality of soil DNA for downstream applications.

Figure 4. Microbial profiles from soil samples (clay and top soil) isolated using Norgen's Soil DNA Isolation Plus Kit (Cat. 64000-NB) and Competitor M's kit. The relative abundance of phylum-level classifications indicates the efficiency of DNA isolation and the qualityof DNA. 16S rRNA genes (v3-4 region) were amplified and the amplicons were sequenced on Illumina® MiSeq™ (MiSeq Reagent Kit v3) using Norgens 16S Metagenomic workflow with Illumina 16S Metagenomics Pipeline (v1.0.1).

Figure 5. Abundance of bacterial genera heat map based on phylum level between two DNA soil samples isolation methods; Norgen's Soil DNA Isolation Plus Kit (Cat. 64000-NB) and Competitor M's kit. The numbers indicate species count.