Plasma/Serum RNA Purification Mini Kit (Spin Columns), 50 - 200 ul sample input

Product Description

• No phenol:chloroform extractions – Circulating RNA and Exosomal RNA is isolated without the use of harmful chemicals such as phenol or chloroform
• Isolate all sizes of circulating RNA and exosomal RNA - The kit allows for the isolation of all sizes of fragmented circulating RNA and exosomal RNA, including microRNA
• Fast and easy processing - Rapid spin column format allows for the processing of multiple samples in under 15-20 minutes..
• Small input volume - Isolate circulating RNA and exosomal RNA from 50 ul to 200 ul of plasma/serum. Concentrate circulating RNA and Exosomal RNA– Circulating and exosomal RNA present in input volumes of 50 ul to 200 ul are concentrated into final elution volume of 10 ul to 25 ul.
• Isolate inhibitor-free RNA - Purified RNA can be used in a number of sensitive downstream applications including reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays

This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR.

The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL and is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Background

Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

Applications

The purified circulating RNA is compatible with most of the molecular biological applications such as:

• Reverse transcription qPCR
• Reverse transcription PCR
• Northern blotting
• RNase protection and primer extension
• Expression array assays.
• miRNA array
• RNA-Seq
• Nanostring
• Fluidigm
• Droplet/Digital PCR