myTXTL Pro Cell-Free Expression Kit
Accelerate cell-free protein expression from linear and plasmid DNA
Product Description
myTXTL cell-free expression kits enable rapid expression of protein targets, with many advantages over in vivo protein expression including the ability to deliver assayable protein in a single day and to skip time-consuming cloning and lysis/purification steps.
- Single-day workflow
- Skip lysis/purification
- No cloning required, use linear or plasmid DNA
- Express toxic proteins
- Industry-leading yields
- Maximum versatility
The myTXTL Pro Kit provides maximal expression of proteins without disulfide bonds, by leveraging the combined advantages of previous myTXTL kits.
Each myTXTL Pro Kit contains the Pro Master Mix, the Helper Plasmid (for T7 RNA polymerase expression), and the T7 deGFP Control Plasmid (for T7-promoter based expression of deGFP).
To express proteins that contain disulfide bonds, please see our myTXTL Antibody/DS Kit.
Applications
- High-throughput protein or enzyme engineering
- Protein synthesis (10-200kDa and higher)
- Production of soluble and membrane proteins
- Rebooting bacteriophages
- Prototyping of gene networks or gene circuits
- Construction of synthetic minimal or artificial cells
- Metabolic pathway optimization
- Metagenomic screening
- Teaching biological concepts
- And more!
Performance
myTXTL Pro Master Mix exceeds the performance of competitor systems
T7 deGFP expression was quantified following default expression protocols for myTXTL Pro Master Mix and several competitor systems. Reactions proceeded for the times indicated below.
Figure 1. Expression of the myTXTL T7 deGFP Control Plasmid was examined in multiple cell-free systems and quantified by deGFP fluorescence. Cell-free expression was conducted for the duration recommended for each system.
A wide range of proteins express at high-yield with myTXTL
Proteins ranging from 12-160 kDa were expressed following the standard myTXTL protocol. 1 μL of each reaction was assayed by SDS-PAGE without purification.
Figure 2. SDS-PAGE visualization of proteins expressed in myTXTL Master Mix using 5 nM Template DNA. 1 µL of myTXTL reaction is visualized per lane.
