myTXTL - Cell-Free Expression

Just add DNA to express analysis-ready antibodies or target proteins in hours!

• Accelerate your workflow -  accomplish in two days what would take 3 weeks with in-vivo methods
• Scale up production with high-throughput solutions
• Achieve superior results with tools designed for ease of use
• Perform protein expression not possible with in-vivo methods

In cell-free protein expression systems, gene transcription (TX) and translation (TL) are unbound from constraints required for cell culture-based processes. myTXTL systems offer a versatile and easy-to-use cell-free expression platform for protein synthesis, enzyme engineering, and synthetic biology processes in industry and academia.

Daicel Arbor's cell-free myTXTL systems allow rapid and parallel production of soluble or toxic proteins and other complex biological structures, like bacteriophages, under open-reaction conditions. This enables easy manipulation, addition of chaperone molecules and straightforward downstream processing of synthesized proteins, enzymes and phages.

Two myTXTL Systems

Choose what's right for you:

• myTXTL Antibody/DS Kit: Engineered for the expression of antibodies and disulfide-bond containing proteins, can also express proteins without disulfide bonds
• myTXTL Pro Kit: High yield system for proteins that do not require disulfide bond formation

myTXTL Workflow

The endogenous transcription (TX) and translation (TL) machinery from E. coli is utilized for myTXTL. Protein expression is initiated by simply adding DNA to the master mix and target protein activity can be measured in as little as 15 minutes.

Introduction to myTXTL Cell-free Protein Expression