Single Cell RNA Purification Kit

Product Description

• Fast and easy processing - The Rapid micro spin-column format allows the processing of 10 samples in 20 minutes
• Small elution volume of 8 µL
• Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without compromising yield
• No phenol or chloroform extractions
• Isolate high quality total RNA from small inputs amounts of a variety of sources
• RNA can be isolated and detected from as little as a single animal cell

Norgen’s Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The special design of the micro spin-column allows a small elution volume of as little as 8 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. Briefly, the cells of interest are first lysed using the provided Lysis Solution, ethanol is added and the RNA is bound to Norgen's Single Cell RNA Spin Column (BIND). Under these conditions only the RNA will bind to Norgen's resin while most of the contaminating cellular proteins are removed in the flowthrough or retained on top of the resin. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into the provided Elution Buffer or water (ELUTE).

Workflow