Endotoxin Removal Kit for DNA (Mini)

Product Description

• Reduce endotoxin levels to 0.1 EU/µg DNA or less
• Effectively remove endotoxins in as little as 20 minutes
• Remove endotoxins from up to 25 µg of DNA
• Rapid spin-column format
• Also available Midi and Maxi format

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA.  Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. This kit reduces endotoxins to 0.1 EU/mg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. The convenient spin column procedure can be completed in approximately 20 minutes and typical recovery of DNA is >90% of the starting sample.  

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications. 

Figure 1. Efficiently Remove Endotoxins