Bacterial Cell Lysis Reagent for Inclusion Body Release

Product Description

• Proprietary solution of detergents, protease-inhibitors and buffer
• Efficient lysis of bacterial cells for extraction of inclusion body proteins

The Cell Lysis Reagent is designed for the gentle and efficient lysis of bacterial cells in order to assist in the extraction of inclusion body proteins.  Cell lysis is accomplished through non-ionic detergent chemical disruption in conjunction with mechanical disruption.  The use of a needle and syringe during the procedure helps to reduce viscosity and facilitate purification, producing proteins that are often greater than 95% pure. 

Cell Lysis Reagent does not solubilize inclusion bodies. Inclusion Bodies can be solubilized using Norgen's Inclusion Body Solubilization Reagent (available separately). Norgen's Inclusion Body Protein Isolation Kits can also be used as complete kits for  cell disruption, inclusion body solubilisation and purification using spin column chromatography.

Performance Data

Figure 1: Efficient Isolation of Inclusion Body Proteins Using Norgen's Cell Lysis Reagent and Inclusion Body Solubilization Reagent. Numbers represent kDa sizes of protein bands. Inclusion Bodies were extracted and solubilized following gene expression. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250.