Bacterial Genomic DNA Isolation Kit (96-well format)

Product Description

• Fast and easy processing using either a vacuum manifold or centrifugation
• Isolate high quality genomic DNA
• Isolate genomic DNA from various inputs including both Gram-negative and Grampositive cultures, including Escherichia coli and Bacillus cereus
• Recovered genomic DNA is compatible with various downstream applications

Norgen’s Bacterial Genomic DNA 96-Well Kit provides a rapid method for the high-throughput
isolation of genomic DNA from 2 x 10⁹ viable bacterial cells (between 0.5 and 1.0 mL of culture).
Purification is based on 96-well column chromatography as the separation matrix. Norgen’s 96-
well plate binds DNA under optimized salt concentrations and releases the bound DNA under low
salt and slightly alkali conditions. The purified genomic DNA is fully digestible with all restriction
enzymes tested, and is completely compatible with downstream applications including real-time
PCR and Southern Blot analysis.

Norgen’s Bacterial Genomic DNA Isolation 96-Well Kit allows for the isolation of genomic DNA
from both Gram-negative and Gram-positive cultures, including Escherichia coli and Bacillus
cereus. The genomic DNA is preferentially purified from other cellular proteinaceous
components. Typical yields of genomic DNA will vary depending on the cell density of the
bacterial culture and the bacterial species. Preparation time for a single 96-well plate is less than
90 minutes, and each kit contains sufficient materials for 192 preparations.