Metabolism & Oxidative Stress
Profiling a new metabolic pathway for your research often means sourcing a different kit for every analyte. One method for glucose, another for lipids, another for energy metabolites. These kits give you substrate-specific results in standard microplate formats. No specialised equipment is needed.
Carbohydrate metabolism kits cover glucose in serum, urine, and cell lysate. Amino acid and protein metabolism kits cover branched-chain amino acids, glutamine, proline, and arginine. Lipid metabolism kits cover fatty acid uptake, triglycerides, and cholesterol. Energy metabolite kits cover ATP, NAD/NADH, NADP/NADPH, and CoQ10. TCA-cycle kits cover succinate, citrate, and malate.
Bioenergetics profiling in disease models
Measure ATP, NAD/NADH ratio, and glucose consumption in your cancer lines or iPSC-derived neurons to map reprogramming linked to the Warburg effect.
Drug target and toxicology screening
CoQ10 level and lactate/pyruvate ratio flag mitochondrial toxicity early, often paired with oxidative stress assays in safety pharmacology panels.
Metabolic disease model characterisation
Quantify hepatic lipid buildup, impaired amino acid catabolism, and elevated NADP/NADPH ratio in your NAFLD and diabetes models from one lysate.
Nutritional and nutraceutical research
Amino acid and energy kits track substrate availability after dietary interventions, supporting your studies on caloric restriction and ketogenic diets.
Immunometabolism and TCA-cycle flux studies
Succinate, citrate, and malate levels track flux during T-cell activation or macrophage polarisation, linking to cell-based assay panels.
Multi-analyte snapshots from limited samples
Combination panels measure three to five metabolites from one lysate, useful for your small biopsies, then normalise with protein kits.
The NAD/NADH ratio and total ATP level are the most direct indicators of mitochondrial oxidative phosphorylation activity. The lactate/pyruvate ratio reflects cytosolic redox state and glycolytic flux. CoQ10 measurement addresses electron transport chain integrity.
Together, these four readouts distinguish between impaired substrate oxidation, electron transport defects, and uncoupling. Full respiratory chain characterisation still requires oxygen consumption rate measurement.
Yes, but you need to optimise the lysis conditions for each analyte. Some metabolites, such as NAD and NADH, are unstable at neutral pH and need immediate stabilisation after lysis.
Combination panel kits are pre-optimised for the same lysis buffer. Using a split-lysate approach, you can measure three to five analytes from a single sample in one run, which reduces the number of cells you need.
Yes. Most colorimetric and fluorometric kits are validated on cell lysates, tissue homogenates, plasma, and serum. Tissue homogenates need thorough mechanical disruption and centrifugation to remove debris before the assay.
Lipid-rich tissues such as liver or adipose tissue may need deproteinisation to reduce background absorbance in colorimetric formats.
The standard approach is to normalise to total protein content, using a Bradford or BCA assay run on an aliquot of the same lysate. Cell count-based normalisation is also acceptable when you count cell numbers precisely before lysis.
DNA content normalisation by fluorometric PicoGreen assay is an alternative. It is less sensitive to differences in cytoplasmic volume between cell types.
Product catalogs
Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.
