Methylated RNA Immunoprecipitation
Methylated RNA Immunoprecipitation
Enrichment and Quantification of RNA fragments
m1A, m6A and m7G are prevalent and reversible post-transcriptional RNA modifications that decorate mRNA, tRNA and rRNA. Recent studies have revealed the crucial roles of m7G RNA modification in multiple cellular processes, including RNA structural stability, folding, interactions with proteins, cell viability, impaired selfrenewal ability, cell proliferation, and cell death. Methylated RNA immunoprecipitation (RIP) is a gold standard method to monitor the status and map the location of RNA modifications in specific genes and in transcriptome wide.
The methylated RNA Enrichment and Quantification Kits from RayBiotech identify the abundance and enrichment location of m1A, m6A or7G by MeRIP and quantitative realtime PCR in a fast and streamlined procedure.
EpigenTek's CUT&RUN m6A MeRIP uses a state-of-the-art RNA cleavage enzyme mix to simultaneously fragment immunocaptured RNA and cleave/remove any RNA sequences in both ends of the target m6A -containing sequences, without affecting RNA regions occupied by the antibody. Short RNA fragments are consequently generated only bound with anti- m6A antibody. True target m6A -enriched regions can therefore be reliably identified, and high-resolution mapping achieved.