The Kits from Daicel Arbor Biosciences provide pools of in-solution biotinylated RNA probes plus reagents for highly-efficient, scalable targeted sequencing on any NGS platform, including Illumina®, Ion Torrent®, PacBio®, and Nanopore®. Daicel Arbor's proprietary oligo synthesis technology delivers high-quality, complex baits at extremely competitive pricing. In addition, we can offer complimentary bait design and project development assistance from a team of expert scientists.
myBaits kits have been successfully used in thousands of research projects from a wide variety of genome types (e.g. animals, plants, viruses and microbes) and DNA sources (e.g. fresh, degraded, and environmental).
Many biological sample types are dominated by DNA from other sources, requiring significant next-generation sequencing (NGS) depths to resolve specific microbial genomes or to fully characterize the variation within microbial communities. Targeted sequencing that excludes background non-target DNA from samples prior to NGS solves this problem and dramatically reduces the overall costs of sequencing and data analysis per sample. Hybridization capture is the most versatile technique for comprehensive, cost-effective targeted NGS of e.g. viruses and bacteria in complex samples.
Kits tailored for specific target regions are the most popular choice, allowing enrichment of specific SNPs, exons, genes, and other sequence motifs from genomic or metagenomic samples.
Daicel Arbor Biosciences also offers a variety of predesigned kit options for specific research applications, such as mitochondrial DNA sequencing, ultraconserved element sequencing, whole-genome enrichment from metagenomic samples, and human cancer exome research.
Figure 1.
(1) HYBRIDIZATION – An NGS library is denatured via heat, and allowed to hybridize to a complex mixture of complementary biotinylated RNA baits over the course of several hours. Adapter-specific blocking oligos prevent random annealing of library molecules at the common adapter sites.
(2) WASHING – After the hybridization is complete, the biotin present on each bait is bound to a streptavidin-coated magnetic bead. Wash steps help remove off-target or poorly-hybridized library molecules.
(3) AMPLIFICATION – The remaining library molecules that are still bound to their complementary baits are denatured via heat, and amplified using universal library primers. This “enriched” library can now be sequenced.
Hybridization capture with myBaits Custom is an extremely versatile and flexible technology that has been applied to an enormous variety of applications and taxa, such as plants, animals, humans, bacteria, and viruses.
Hundreds of studies have been published with myBaits over the years. Across the wide variety of applications, myBaits Custom kits frequently achieve high on-target read percentages and/or high unique read complexity for most applications, creating many orders of magnitude savings in sequencing compared to shotgun approaches.
In addition, research scientists are continually innovating novel improvements to the speed, ease of use, and performance of our myBaits kits across a variety of applications.
We can always provide specialized experimental design advice to maximize success with your next NGS targeted sequencing project. Our Sequence Submission Guidelines describe how you need to provide your sequence data for the design of your myBait set.
Figure 2. NGS reads from enriched human gDNA library. Single-end NGS reads are shown aligned to the hg38 genomic reference sequence. Positions of the four 80nt myBaits probes in this region are indicated by blue bars. Unique read coverage is highest across the baited region, and tapers off upstream and downstream of the baited region. If sequencing further into the known or unknown flanking regions is desired, simply increase the length of your NGS library molecules (compatible with any myBaits kit).