Single-cell RNA sequencing, spatial transcriptomics and multiomics with SeekGene
Explore gene expression, immune repertoire, chromatin accessibility, DNA methylation and spatially resolved transcriptomics at single-cell or single-nucleus resolution. SeekGene combines compatible SeekOne™ DD digital droplet workflows with application-specific reagents, while the SeekSpace™ workflow adds spatial information from tissue sections. The right solution depends on your research question, sample format and required molecular readout.
Choose the right SeekGene workflow
| Workflow | Research question / application | Key selection point |
|---|---|---|
| SeekOne DD 3' scRNA-seq | Cell populations, gene-expression profiles and cellular heterogeneity. | 3' gene-expression profiling from suitable cell/nucleus preparations; not for FFPE tissue. |
| SeekOne DD 5' RNA + immune profiling | Transcriptomics alongside TCR/BCR immune repertoire analysis. | 5' gene expression with compatible receptor-enrichment reagents; immune profiling is subject to human/mouse and sample restrictions. |
| SeekOne DD scFAST-seq | Broad, near-full-length RNA coverage, transcript structure and alternative splicing. | Random-primed workflow; check species compatibility of the rRNA-blocking reagents. |
| SeekOne DD scMLPseq | Targeted gene-expression analysis from suitable FFPE material. | Probe-based assay; target coverage follows the probe panel, with current manufacturer support for human samples. |
| SeekOne DD Multiome ATAC + RNA | Chromatin accessibility and gene expression in matched nuclei. | Combined epigenomic and transcriptomic readout; nuclei input required. |
| SeekOne DD Multiome Methylation + RNA | DNA methylation together with gene expression in the same cell. | Combined methylome/transcriptome workflow with assay-specific sequencing-depth requirements. |
| SeekSpace™ Single Cell Spatial Transcriptome-seq | Gene expression linked to spatial location within a tissue section. | For OCT-embedded fresh-frozen tissue sections; combines spatial labelling with SeekOne DD processing. Not for FFPE. |
SeekOne DD and SeekSpace: complementary workflows
The SeekOne™ DD Digital Droplet System supports microfluidic partitioning and barcoding in compatible single-cell and single-nucleus assays. SeekSpace™ extends the portfolio to spatial transcriptomics: spatially labelled nuclei from a tissue section are processed with the compatible SeekOne DD system to connect expression data with location in the original tissue. Unlike conventional dissociated-cell RNA-seq, this workflow retains information about spatial organisation.
Explore the SeekSpace kit and product portfolio
For spatially resolved transcriptomics, see the SeekSpace™ Single Cell Spatial Transcriptome-seq Kit. For a broader overview of assays, instrument requirements and sample formats, consult the SeekOne™ DD Series product brochure. Compare the available reagents below and check the product-specific requirements before ordering.
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