Reporter gene assays for promoter activity, signalling pathway, and compound response readouts
When you need quantitative readout of promoter activity, transcription factor binding, signalling pathway state, or compound response, reporter gene assays give the readout in 96- or 384-well format. Foundational to functional genomics and pharma compound profiling.
Pick reporter by readout.
- Highest dynamic range. Firefly luciferase.
- Kinetic time-course on live cells. Secreted Gaussia.
- Dual-reporter internal normalisation. Firefly-plus-Renilla or GLuc-plus-SEAP.
- Brightest at small size. NanoLuc.
- Counter-screen for compound screens. Always include counter-screen on non-responsive reporter to filter promiscuous luciferase inhibitors.
GeneCopoeia leads with Secrete-Pair Dual Luminescence and pEZX vectors. Elabscience provides ELISA-format equivalents.
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