Protein Purification

Protein extraction and subcellular fractionation kits for clean, unbiased samples

Spin-column-based total protein extraction uses a proprietary resin to recover proteins across a broad molecular weight range, giving you clean starting material for proteomics, structural studies, or functional assays regardless of extraction method. The column format lyses cells or tissues in under one minute, without sonication or RIPA buffer, and pairs with the Precellys tissue homogenisers when tissue needs breaking up before lysis. This approach eliminates non-systematic protein loss, helps preserve post-translational modifications, and delivers a complete, unbiased protein profile compatible with western blot, mass spectrometry, and protein quantitation assays. Subcellular fractionation kits isolate specific compartments, including plasma membrane, cytosolic, mitochondrial, and nuclear fractions, without ultracentrifugation, with formats for animal cells, solid tissues, plant material, and specialised matrices such as blood vessels.

Selection turns on five questions: starting material (cultured cells, mammalian tissue, plant, microbe, serum each have specific lysis chemistries), target compartment (whole-cell lysate is fastest; subcellular fractionation isolates specific organelles; secreted-protein purification needs conditioned-media handling), tag or capture chemistry (His for routine; FLAG or HA for stringent immunoprecipitation; Strep for low background; antibody-based for endogenous proteins), downstream compatibility (mass spec needs detergent-free or MS-compatible buffers; activity assays need native conditions), and scale (microgram for analytical work versus milligram for structural biology). Invent Biotechnologies specialises in detergent-free subcellular fractionation; Norgen Biotek covers serum and biofluid protein extraction; Elabscience offers general lysis and IP reagents. Match buffer chemistry to your downstream assay before choosing a kit.