Library Quantification

qPCR-based NGS library quantification kits for accurate pool balancing and cluster prediction

When you need accurate NGS library quantification for pool balancing on the sequencer, qPCR-based kits measure functional (sequenceable) library molecules rather than total DNA. Outperforms fluorometric (Qubit) and electrophoretic (Bioanalyzer) methods for accurate cluster generation prediction.

Pick by platform.

1. Illumina. Standard qPCR-based library quant.
2. Element and MGI. Platform-specific qPCR primer sets.
3. Caveat. For multiplex sequencing always use qPCR-based library quant for pool balancing. Fluorometric systematically over-estimates functional library and leads to over-clustered flow cells.
4. Throughput. Match to your library batch size.

Meridian (Bioline) supplies library quantification kits.