Lentiviral Packaging

Lentiviral packaging plasmid systems for stable transgene delivery in HEK293T cells

When you need to produce third-generation HIV-derived lentivirus particles for stable transgene delivery, packaging plasmid systems handle gag-pol, envelope, and rev in matched combinations. Standard packaging in HEK293T producer cells.

Pick by safety and tropism.

1. Generation. Third-generation is safety standard. Second-generation simpler with one fewer plasmid.
2. Envelope. VSV-G for broad tropism. Alternative envelopes for cell-type targeting.
3. Titre. Scale to 10-cm dishes or larger.
4. Titration. qPCR-based titration; UV overestimates infectious titre by 3- to 10-fold.
5. Production. HEK293T standard producer.

Cellecta supplies packaging mixes in this inventory.