Exosome Release & Transfer

NLuc-based EV reporter tools and secretion modulators for quantifying exosome release and uptake

Reagents that selectively modulate EV secretion let you distinguish exosome-mediated effects from direct cell contact or soluble factor signalling when studying biogenesis or intercellular cargo transfer. Bioluminescent reporter systems fuse a nanoluciferase tag to EV surface markers such as CD9, CD63 or CD81, giving a real-time readout of secretion rates and recipient-cell uptake on any standard plate luminometer. Three reagent formats support your workflow: lentiviral expression vectors build stable EV reporter cell lines, pre-built stable HEK293 reporter lines give an immediate starting point, and purified EV reporter preparations add directly to recipient cells for uptake studies. EV-Luminite reagents are available with NLuc fused to CD9, CD63 or CD81, alongside secretion modulation reagents for pharmacological inhibition or stimulation of EV biogenesis.

Pick by readout.

1. Bulk-population kinetics. Choose luciferase reporters fused to tetraspanins. Real-time secretion on a plate reader.
2. Single-cell imaging. Choose fluorescent reporters.
3. Endpoint cargo quantification. Choose ELISA.
4. Tag location. CD63 fusion for tetraspanin-labelled exosomes. Lipid-anchored for non-tetraspanin populations.
5. Caveat. Confirm reporter expression and exosome localisation by Western and immunoprecipitation. Tag fusions can alter biogenesis kinetics.

SBI System Biosciences supplies the Cyto-Tracer platform.