Exosome Antibodies & Antibody Arrays

Exosome marker antibodies for CD63, CD81, CD9, TSG101 and ALIX detection

Characterising extracellular vesicle populations calls for antibodies against tetraspanins CD9, CD63 and CD81, alongside ESCRT-pathway markers TSG101 and ALIX, to build the ISEV-recommended multi-marker panel. Bundled kits supply rabbit antibodies against CD9, CD63, CD81 and Hsp70 in a single purchase, reducing lot-to-lot variability across your four-marker panel. Individual rabbit antibodies against TSG101 and Vimentin are validated for human, mouse and rat samples and pair with a goat anti-rabbit HRP secondary for Western blot. Multiplex antibody arrays extend single-marker ELISA to simultaneous profiling of surface proteins across multiple EV subpopulations.

Pick by panel depth.

1. Minimum panel. Canonical tetraspanins (CD9, CD63, CD81). Publication standards typically require two positives plus TSG101 or ALIX.
2. Expanded panel. Add TSG101, ALIX, HSP70 plus lineage-specific surface markers for subtype discrimination.
3. Application format. Western blot, flow cytometry, ELISA, or antibody array. Match to throughput and sample.
4. Validation. Verify antibody performance in EV context. Off-the-shelf antibodies sometimes underperform on exosomes.
5. Multiplex. Use exosome antibody arrays for parallel measurement of multiple markers.

SBI System Biosciences carries the EV-specific panels.