DNA-seq from Genetic Screens

DNA-seq library prep reagents for pooled CRISPR and shRNA screen analysis

When you have run a pooled CRISPR or shRNA screen, DNA-seq reagents handle the library prep from genomic DNA through barcode-count tables. Includes screen-specific PCR enzymes, indexing primer sets matched to lentiviral vector backbones, and library quantification controls.

Pick by screen and PCR strategy.

1. Screen format. Indexing primer set must match the lentiviral backbone of the library. Mismatch leads to no amplification.
2. PCR strategy. One-step PCR with indexing primers is fastest. Two-step PCR adds index purity and lowers PCR-bias for low-input samples.
3. Library quantification. qPCR-based gives more accurate sequencing-pool ratios than fluorometric or Bioanalyzer methods.
4. Coverage. For low-input or rare-event positive-selection screens use two-step PCR to retain library complexity.
5. Indexing scheme. UDI for low cross-talk at scale.

Cellecta supplies library-matched reagents for DECIPHER screen platforms.