CUT&Tag Assays

CUT&Tag assays for streamlined, low-input chromatin profiling in one tube

When you want streamlined chromatin profiling in one tube, CUT&Tag uses a protein-A-Tn5 transposase fusion that inserts sequencing adapters directly at the antibody-binding site. From antibody binding to sequencing-ready library in one tube, with very low cell-input requirements (down to single-cell).

Pick by input and species.

1. Enzyme platform. pA-Tn5 standard. pAG-Tn5 covers broader antibody species range.
2. Cell input. CUT&Tag works from 1,000 cells. Single-cell variants exist.
3. Antibody validation. Poor antibody quality dominant failure mode. Validate with SNAP-CUTANA spike-in nucleosomes before scaling.
4. Bridge antibodies. For uncommon IgG species.
5. Bioinformatics. Specialised pipelines for Tn5-inserted libraries.

EpiCypher dominates with CUTANA pAG-Tn5 kits and matched antibody panels.