CUT&Tag assays for streamlined, low-input chromatin profiling in one tube
CUT&Tag (Cleavage Under Targets and Tagmentation) profiles histone post-translational modifications directly in cells or nuclei. Target-specific antibodies recruit pAG-Tn5, a protein A/G–Tn5 fusion that inserts sequencing adapters near antibody-bound chromatin. The CUTANA™ Direct-to-PCR workflow produces sequencing-ready libraries without a separate conventional library-preparation process.
CUTANA CUT&Tag assays are optimized for histone marks. The standard workflow recommends 100,000 native nuclei per reaction and has been validated down to 10,000 nuclei for selected targets. Sample requirements depend on the target and protocol; single-cell, spatial and multiomic variants require dedicated workflows.
Choose the components for your CUT&Tag experiment
| Component | When to choose it | Selection point |
|---|---|---|
| CUTANA™ CUT&Tag Kit | Starting a complete, standardized CUT&Tag workflow | One-tube Direct-to-PCR assay with pAG-Tn5, buffers, indexing and purification reagents, plus defined positive, negative and spike-in controls. Check the current kit version and primer-set configuration. |
| CUTANA™ pAG-Tn5 | Building or maintaining an established DIY CUT&Tag protocol | Ready-to-use, adapter-loaded pAG-Tn5 for antibody-directed tagmentation; other buffers, primers and controls must be selected separately. |
| CUT&Tag-validated antibodies and secondary antibodies | Profiling a specific histone modification | Choose primary antibodies validated for CUT&Tag where possible and check the required species/isotype-specific secondary antibody. |
| SNAP-CUTANA™ K-MetStat Panel | Assay QC and quantitative histone-methylation studies | DNA-barcoded nucleosome spike-ins help assess assay performance, on-/off-target PTM recovery and normalization for supported methylation targets. |
| Supporting reagents and clean-up products | Supplementing existing or customized workflows | Select appropriate beads, PCR reagents and other components according to the kit or DIY protocol; contents may already be included in a complete kit. |
Why use CUTANA CUT&Tag?
The streamlined Direct-to-PCR protocol supports efficient chromatin profiling, with 8-strip-tube and multichannel-pipetting compatibility. The CUTANA workflow can generate sequencing libraries in approximately two days; EpiCypher recommends around 5–8 million sequencing reads per sample for its optimized histone-PTM assays. Requirements and performance depend on the experiment and the relevant protocol.
Controls, protocols and related methods
Include appropriate positive and IgG negative controls to assess signal and background. For supported histone lysine-methylation targets, SNAP-CUTANA spike-in controls provide an additional experimental quality check. Match antibodies and controls to the target rather than assuming one panel validates every antibody.
For chromatin-associated proteins such as transcription factors, consider CUT&RUN instead. To compare CUT&Tag with other chromatin-mapping approaches, visit the BioCat Epigenomic Profiling overview. For current experimental details and kit-version-specific instructions, consult the EpiCypher CUT&Tag protocols and the manuals linked on the relevant product pages.
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