cDNA synthesis kits with engineered reverse transcriptases for qPCR, sequencing, and cloning
Reverse transcription kits produce complementary DNA from RNA template for downstream qPCR, sequencing, or cloning. The category includes random-hexamer-primed, oligo-dT-primed, and gene-specific-primed reactions, plus engineered reverse transcriptases (RNase H-minus, thermostable, processive variants) tuned for different starting RNA quality and length requirements.
Pick by priming and enzyme.
- Priming strategy. Random hexamer for unbiased coverage. Oligo-dT for poly-A mRNA only. Gene-specific for single-target qPCR.
- Enzyme. RNase H-minus for longer products. Thermostable for difficult-secondary-structure RNA. Processive for full-length first-strand for NGS.
- Input range. Nanogram to microgram inputs. Low-input kits for single-cell or biofluid RNA.
- Downstream compatibility. qPCR-ready versus library-prep-ready. Match to your next step.
- Processivity. For long-read sequencing prep choose highly processive thermostable enzyme; standard cDNA enzymes drop off at 2 to 3 kb.
Meridian (Bioline) covers the standard SensiFAST family. GeneCopoeia offers high-fidelity custom-applications cDNA.
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