Barcode Adapters, Blockers, Clean-Up, Size Selection & Normalisation

Indexing adapters, blockers, clean-up and normalisation reagents for reproducible NGS library prep

When you are doing high-throughput NGS library prep, this section covers indexing adapters, hybridisation blockers, magnetic-bead clean-up, automated size-selection reagents, and library normalisation reagents. Standardised, validated consumables drive reproducibility across batches.

Pick by index scheme and platform.

1. Index scheme. UDI for low cross-talk at scale. Single-index or combinatorial dual-index for less stringent multiplexing.
2. Workflow stage. Adapter, blocker, clean-up, size-selection, normalisation each have specific products.
3. Platform. Illumina-compatible standard. Element and MGI have specific requirements.
4. Bead chemistry. Match to your fragment-size targets.
5. Caveat. For pooled libraries at high multiplexing UDI eliminates a category of cross-talk error; the cost premium is justified.

Meridian Life Science and EpigenTek lead the catalogue.