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Antibody Expression

High-throughput recombinant antibody production, from gene to purified antibody in weeks

When you need antibodies quickly and animal-free, recombinant production gives you the direct route. This high-throughput service takes you from an antibody gene sequence to purified antibody in HEK293 or CHO cells, skipping immunisation and hybridoma screening. Many projects finish in a few weeks, with the delivered amount guaranteed to you up to the milligram level. Multiple isotypes and formats are available to you, and large numbers of antibodies can run in parallel.

What you can order

  1. Isotype. Mouse IgG1 and IgG2a, human IgG1 and IgG4, and rabbit IgG are all supported.
  2. Format. Full-length IgG, or smaller Fab, scFv, and VHH formats. Fab, scFv and VHH are available with a His tag or, alternatively, with an Fc tag.
  3. Scale. From 0.2 mg upward, with larger amounts on request.
  4. Starting point. You send your antibody sequence, and gene synthesis, cloning, transient expression, and purification are handled from there.

Applications

Assay and diagnostic development

Defined recombinant antibodies give reproducible reagents for your ELISA, lateral flow, and immunodiagnostic assays.

Research detection reagents

Sequence-defined antibodies replace variable hybridoma lots, ready to use next to catalogue primary antibodies.

Antibody engineering

Fab, scFv, and VHH formats support format conversion, affinity work, and fusion designs from one sequence.

Discovery follow-up

Hits from phage display or single-cell screens are expressed and purified for validation.

Animal-free production

Recombinant expression avoids immunisation, which suits ethical, reproducibility, and supply-continuity needs.

Parallel panel generation

Many antibodies express at once, which suits screening panels and structure-function studies. Compare formats with research antibodies.

Custom Antibody Production Pricing

Antibody Isotype Quantity Starting Price*
Mouse IgG2a 0.2 mg 495 €
Mouse IgG2a 0.5 mg 575 €
Mouse IgG2a 1 mg 690 €
Mouse IgG2a > 1 mg Please inquire
Human IgG1/IgG4 0.2 mg 495 €
Human IgG1/IgG4 0.5 mg 575 €
Human IgG1/IgG4 1 mg 690 €
Human IgG1/IgG4 > 1 mg Please inquire
Mouse IgG1 0.2 mg 495 €
Mouse IgG1 0.5 mg 575 €
Mouse IgG1 1 mg 690 €
Mouse IgG1 > 1 mg Please inquire
Rabbit IgG 0.2 mg 495 €
Rabbit IgG 0.5 mg 575 €
Rabbit IgG 1 mg 690 €
Rabbit IgG > 1 mg Please inquire
Fab/scFv/VHH (His tag) 0.2 mg 575 €
Fab/scFv/VHH (His tag) 0.5 mg 690 €
Fab/scFv/VHH (His tag) 1 mg 895 €
Fab/scFv/VHH (His tag) > 1 mg Please inquire

*If Fab, scFv and VHH are FC tag, the price is the same as for Rabbit IgG.

Contact BioCat

For detailed pricing, please simply send us your antibody production request

Upon receipt we will closely inspect your project and send you a detailed quote right away. We typically respond within one business day.

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Frequently asked questions

Which isotypes and formats can you make?

Mouse IgG1 and IgG2a, human IgG1 and IgG4, and rabbit IgG are supported, as well as Fab, scFv, and VHH formats with His or Fc tags.

How pure are the antibodies?

Purity is above 95% by SEC-HPLC and above 95% by SDS-PAGE, with endotoxin below 1 EU/mg. A certificate of analysis is included.

How long does a project take?

Many projects complete in a few weeks, from a supplied sequence through to purified antibody, using high-throughput gene synthesis and transient expression.