Ready-to-Use Lentiviral Packaging Plasmid Mix (HIV) (Second- and Third-Generation, psPAX2/pMD2.G based)

Cellecta’s Ready-to-Use Lentiviral 2-Plasmid Packaging Mix allows production of VSV-G pseudotyped viral particles when co-transfected into packaging cells together with second- or third-generation HIV-based lentiviral vectors.

The Lentiviral Packaging  Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. 

These plasmids provide all the necessary structural, regulatory, and replication proteins required to produce pseudotyped packaged lentiviral expression constructs when co-transfected with an HIV-based lentiviral expression construct (e.g., Cellecta pRSG sgRNA expression construct) into a producer 293T cell line (e.g., ATCC, Cat.# CRL-11268™). Pseudotyped lentiviral particles with VSV-G envelope protein can infect a variety of mammalian or non-mammalian, dividing or non-dividing cells.