SKU: MA-0135-AKR
Myeloperoxidase Activity Assay Kit
In Stock
Blue Ice
Microplate reader, Colorimetric, OD 412 nm
Specifications
| Specification | Details |
|---|---|
| Sensitivity | <0.05 mU/well |
| Application | To measure the enzymatic activity of MPO in a wide range of sample types. |
| Additional Information | Myeloperoxidase (MPO) is an oxidative enzyme abundantly expressed in neutrophils and is involved in the immune response and inflammation. Additionally, MPO can also oxidize lipids, proteins and DNA, leading to oxidative damage in chronic inflammatory conditions such as atherosclerosis, COPD and neurodegenerative disease. AkrivisBio’s MPO Assay is a simple sensitive means of measuring MPO enzyme activity below 0.05 mU per well, which can be indicative of neutrophil activation and oxidative stress. In the assay, Myeloperoxidase produces HOCl (hypochlorous acid) from hydrogen peroxide and chloride. HOCl reacts with taurine forming taurine chloramine, which then reacts with TNB, the reduced form of DTNB, Ellman’s Reagent, bleaching out the color causing a decrease of absorbance measured at OD 412 nm. The absorbance is inversely proportional to the amount of MPO enzyme. |
| Detection Method | Microplate reader, Colorimetric, OD 412 nm |
| Sample Type | Cell Lysate, Tissue Extracts, Urine, Serum, Other biological fluids |
| Assay Category | Oxidase/Peroxidase Activity |
Resources
PDF
Manual
PDF
Brochure
Catalog Number
MA-0135-AKR
Name
Myeloperoxidase Activity Assay Kit
Price(10000 wells)
Pack Size
10000 wells