SKU: EA100587-OR
Mouse IL-12(p40) ELISA Kit
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Sample Size | 100 ul |
| Sensitivity | <10pg/ml |
| Detection Range | 15.6pg/ml-1000pg/ml |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Target Name | Il12b |
| Gene ID | 16160 |
| Specificity | This kit is used for quantitative detection of mouse IL-12(p40) |
| Target Background | Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. |
| Accession No. | NM_001303244 |
| Assay Time | 3.5 hours incubations; 1 hour washing and analyzing samples |
Resources
PDF
Manual
Catalog Number
EA100587-OR
Name
Mouse IL-12(p40) ELISA Kit
Price(1 x 96 Well Plate(s))
Pack Size
1 x 96 Well Plate(s)