SKU: EA100715-OR
Human SPARC ELISA Kit
In Stock
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Sample Size | 100 ul |
| Sensitivity | <20pg/ml |
| Detection Range | 0.78ng/ml-50ng/ml |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Target Name | SPARC |
| Gene ID | 6678 |
| Specificity | This kit is used for quantitative detection of human SPARC |
| Target Background | SPARC(secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus(domain I), 2) a cysteine- rich(domain II), 3) a hydrophilic region(domain III) and 4) an EF hand motif at the carboxy terminus region(domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found. |
| Accession No. | NM_003118 |
| Quantity Group ID | EA100715-OR |
| Assay Time | 3.5 hours incubations; 1 hour washing and analyzing samples |
Resources
PDF
Manual
Catalog Number
EA100715-OR
Name
Human SPARC ELISA Kit
Price(100 plate(s))
Pack Size
100 plate(s)