SKU: EA100221-OR
Human sFAS ELISA Kit
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Sample Size | 100 ul |
| Sensitivity | <3pg/ml |
| Detection Range | 31.2pg/ml-2000pg/ml |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Target Name | FAS |
| Gene ID | 355 |
| Specificity | This kit is used for quantitative detection of human FAS |
| Target Background | Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. |
| Accession No. | NM_000043 |
| Assay Time | 3.5 hours incubations; 1 hour washing and analyzing samples |
Resources
PDF
Manual
Catalog Number
EA100221-OR
Name
Human sFAS ELISA Kit
Price(1 x 96 Well Plate(s))
Pack Size
1 x 96 Well Plate(s)