SKU: EA102892-OR
Human GM CSF (CSF2) ELISA KIT
In Stock
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Sensitivity | <1pg/ml |
| Detection Range | 15.6pg/ml-1000pg/ml |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Target Name | CSF2 |
| Gene ID | 1437 |
| Specificity | This kit is used for quantitative detection of Human GM-CSF |
| Target Background | Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. |
| Accession No. | NM_000758 |
| Quantity Group ID | EA102892-OR |
| Assay Time | 3.5 hours incubations; 1 hour washing and analyzing samples |
Resources
PDF
Manual
Catalog Number
EA102892-OR
Name
Human GM CSF (CSF2) ELISA KIT
Price(100 plate(s))
Pack Size
100 plate(s)