SKU: EA101068-OR
EBV-VCA IgM ELISA Kit
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Sample Size | 10 ul |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Specificity | Natural and recombinant human EBV-VCA IgM |
| Target Background | The Epstein-Barr Virus (VCA) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids errors in results. Microtiter wells as a solid phase are coated with inactivated Epstein-Barr viral capsid antigen. Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation Epstein-Barr viral capsid antigen-specific antibodies of specimens where analyte is present and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in cases where analyte is found) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Epstein-Barr viral capsid antigen-specific IgM antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader. |
| Assay Time | 3 hours |
Resources
PDF
Manual
Catalog Number
EA101068-OR
Name
EBV-VCA IgM ELISA Kit
Price(1 x 96 Well Strip Plate(s))
Pack Size
1 x 96 Well Strip Plate(s)