SKU: EA101062-OR
CMV IgG ELISA Kit
In Stock
Blue Ice
Colorimetric
Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Sample Size | 10 ul |
| Design Principle | Sandwich |
| Detection Method | Colorimetric |
| Specificity | Natural and recombinant human CMV IgG |
| Target Background | The Cytomegalovirus (CMV) IgG ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Microtiter wells as a solid phase are coated with inactivated grade 2 Cytomegalovirus (CMV) antigen (strain AD 169). Diluted specimens and ready-for-use controls are pipetted into these wells. During incubation Cytomegalovirus (CMV)-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgG antibodies are dispensed into the wells. During a second incubation this anti IgG conjugate binds specifically to IgG antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Cytomegalovirus (CMV)-specific IgG antibody in the specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader. |
| Quantity Group ID | EA101062-OR |
| Assay Time | 3 hours |
Resources
PDF
Manual
Catalog Number
EA101062-OR
Name
CMV IgG ELISA Kit
Price(100 plate(s))
Pack Size
100 plate(s)