axoCells Human iPSC-Derived Sensory Neuron Maximizer Kit (Cells, Media, Supplement and Coating), male donor, min. 0.5M cells

Product Description

• Cells Derived from human iPSCs
• Convenient kit format containing cells, media, supplement and coating
• Expression of the key nociceptive ion channels including Nav1.7, Nav1.8, Nav1.9, TRPV1, TRPM8 and TRPA1
• Assay-ready in just 21 days
• Functional relevance across multiple assays including capsaicin and menthol treatment, thermoception and neurite outgrowth with paclitaxel
• Designed for advanced in vitro model formats including co-culture, microfluidics devices and organ-on-chip platforms

hiPSC-Sensory Neuron Progenitors have been put together with the new optimized media including the MATURATION MAXIMIZER supplement with the required growth factors in one kit with an easy-to-follow protocol to help you generate the best sensory neuron culture for your research.

MATURATION MAXIMIZER is a fully defined medium supplement for enhancing the existing Sensory Neuron Maintenance Medium. It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.

iPSC-derived sensory neurons treated with the Maximizer express key markers, such as TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8. Kinetic states of e.g. TRPv1 are in a fast response inward rectifying state, to agonists such as Capsaicin. Neuronal responses to capsaicin occur at 1μM for 90% of the sensory neuron population.

The MATURATION MAXIMIZER supplement makes the Human iPSC-derived sensory neuronal culture a more physiological relevant model.

Kit Content

• axoCells hiPSC-derived Sensory Neurons (≥0.5 million cells)
• Sensory Neuron Maintenance Media (250ml)
• Sensory Neuron Maximizer Supplement (1 ml)
• Neural Plating Media (30ml)
• GDNF (10 µg)
• BDNF (10 µg)
• NGF (20 µg)
• NT-3 (10 µg)
• SureBond-XF Xeno-free Coating Reagent (1ml)