Blue-Color AP Staining Kit

Product Description

Alkaline Phosphatase (AP) is a universal pluripotent marker for all types of pluripotent stem cells including embryonic stem cells, embryonic germ cells, and induced pluripotent stem cells. The pluripotent status of stem cells can be characterized by a high level of AP expression, along with the expression of multiple pluripotency markers including the transcription factors Nanog, Oct4, Sox2, stage-specific embryonic antigens, SSEA-1, -3, -4, and tumor related antigens, TRA-1-60, TRA-1-81.

Alkaline phosphatase (AP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides and proteins under alkaline conditions. The Blue Color Alkaline Phosphatase Staining Kit is a histochemical assay for use on cells grown in tissue culture wells or dishes. It uses a solution containing substrate and color dye as the colorimetric read-out for the enzymatic activity. The AP-positive, undifferentiated stem cells stain blue. The stain develops quickly, does not readily fade, and can be analyzed 15 to 30 minutes after application to the cultured cells. For added convenience, the stained samples can be kept at 4°C for up to one week for analysis.