Rapid-Lenti HIV-1 p24 ELISA Kit

The Rapid-Lenti HIV-1 p24 ELISA Kit (Single Wash) can be used to monitor HIV-1 in cell culture or to determine the viral titer of lentiviral samples and can be completed in as little at 90 minutes. Microtitration wells coated with murine anti-HIV-1 p24 capture antibody are exposed to test specimens, which may contain HIV-1 p24 reactive determinants. A mixture of biotinylated anti-HIV-1 p24 antibodies and streptavidin HRP conjugate are then added forming a complex (HIV-1 p24/antibodies/conjugate) that binds to the wells. After an incubation period, unbound components in the test sample are washed away. Specifically bound enzyme conjugate is detected by reaction with the Substrate Solution, tetramethylbenzidine (TMB). The assay is measured spectrophotometrically to indicate the level of HIV-1 p24 reactive determinants present in a sample.