VitroGel ORGANOID Hydrogel 3

VitroGel® ORGANOID are xeno-free hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs).

Product Description

VitroGel ORGANOID hydrogels are ready-to-use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

• For organoid culture
• Ready-to-use
• Xeno-free
• Injectable
• Biocompatible
• Work at room temperature

The hydrogels can work together with VitroGel STEM, a hydrogel system for 3D static suspension cultures and scale-up of human pluripotent stem cells, by transferring the stem cell spheroids from VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. The key growth factors and molecules can directly mix with the hydrogel matrix or add on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution. VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

3D cell culture process in 20 min – "Just add cells"

VitroGel ORGANOID is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Which VitroGel ORGANOID Hydrogel should I choose for my organoid culture type?

Because there is a wide range of organoid cell resources from stem cells, patient-derived tissue, co-culture, and PDX, it is hard to tell which organoid hydrogel would perform the best for your experiment. Therefore, the VitroGel ORGANOID Discovery Kit helps you screen the four different formulations to determine the best organoid hydrogel version for your organoid conditions.

The VitroGel ORGANOID Discovery Kit includes the four different formulation types of VitroGel ORGANOID hydrogels: VitroGel ORGANOID 1, VitroGel ORGANOID 2, VitroGel ORGANOID 3, and VItroGel ORGANOID 4

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions. From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids. By saying that, it is still suggested to use the Discovery Kit to make a quick screening to find the optimal hydrogel for your organoid conditions.

VitroGel ORGANOID system can culture organoids in variety of methods:

3D DOME  |  2D DOME  |  2D Hydrogel Coating  |  3D Cell Encapsulation  |  3D Hydrogel-Cell Droplet

Easy cell recovery from hydrogel

Cells cultured in this system can be easily harvested out with our VitroGel Organoid Recovery Solution in 20 minutes.