Immortalized Human Cranial Periosteal Cells (Tag58)

Immortalized Human Cranial Perisoteal Cells (Tag58) is the first human cranial periosteal cell line isolated from healthy adult patient biopsy and immortalized by lentiviral transduction with the large SV40 T antigen. Tag58 cells maintain long-term cell proliferation and osteogenic differentiation potential showing stronger mineralization. Tag58 cells express high levels of stem cell surface markers, CD146, and MSCA-1 (mesenchymal stem cell antigen-1), as well as high constitutive mRNA levels of key factors of osteogenesis. Tag58 cell line represents a suitable experimental platform for studying periosteal cell biology, bone formation, and bone regeneration.